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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was discovered to affiliate with, and be involved with, the phosphorylation of NPAT protein (nuclear protein mapped to the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a essential function in promoting mobile-cycle progression from the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have been described. Two more splice variants were noted but thorough nucleotide sequence information isn't nevertheless obtainable.
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Blocking peptides are peptides that bind particularly into the goal antibody and block antibody binding. These peptide usually consists of the epitope recognized by the antibody.
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This guide outlines even more achievable optimization of mobile and immunoassay parameters to be sure the best possible results are...
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This manual outlines further possible optimization of mobile and immunoassay parameters to be sure the best possible benefits are...
Antibodies sure to the blocking peptide no longer bind towards the epitope on the goal protein. By evaluating the staining from your blocked antibody vs . the antibody alone, you can see which staining is certain.
Concentrate on Data Cyclin E belongs to the remarkably conserved cyclin loved ones, whose users are characterized by a remarkable periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Distinctive cyclins show distinct expression and degradation styles which contribute for the temporal coordination of every mitotic party. Cyclin E forms a complex with and functions being a regulatory subunit of CDK2, whose exercise is necessary for mobile cycle G1/S changeover. This protein accumulates at the G1-S phase boundary and it is degraded as cells progress by way of S stage.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by both equally antibodies...
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